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ypjD [2019-02-19 20:12:34]
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ypjD [2019-02-19 20:12:34]

putative pyrophosphatase
locus
BSU22500
pI
4.95
mw
12.83 kDa
protein length
111 aa Sequence Blast
gene length
336 bp Sequence Blast
function
unknown
product
putative pyrophosphatase
essential
no
synonyms
jojD

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    2,360,155 2,360,490

    The protein

    Protein family

  • 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)
  • Structure

  • [PDB|2GTA]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
    description
    [Pubmed|20308541]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
  • regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
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    Biological materials

    Mutant

  • BKE22500 ([gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGAACGAAACCTCCTAT, downstream forward: _UP4_GAAGGAAAGTAGAGGAGATC
  • BKK22500 ([gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGAACGAAACCTCCTAT, downstream forward: _UP4_GAAGGAAAGTAGAGGAGATC
  • lacZ fusion

  • pGP2437 (in [protein|search|pAC7]), available in [SW|Jrg Stlke]'s lab
  • References

    Operon and expression

  • 20308541,23894131